product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-HFE
catalog :
LS-C7394
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human, rat
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
8135
AntibodyName :
LS-C7394
TargetSpecies :
rat
Host Species :
rabbit
Product Name :
Anti-HFE
Specificity :
The rat sequence is 100% conserved in rat HFE protein homolog RT1-CAFE (360-aa), HFE splice variants delE2 (14E4) (172 aa), 83% in mouse, 72% in rhinoceros and 61% in human HFE. We recommend the use for human HFE. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity.
ClonalityDesc :
polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.4, 0.1% sodium azide, 40% glycerol.
ImmunogenDesc :
An 18-aa peptide sequence within the C-terminus of rat HFE (1) was synthesized, coupled to KLH.
PurificationDesc :
Immunoaffinity Chromatography
RecommendedStorageDesc :
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
IsotypeName :
IgG
gene :
HFE
StandardGeneSymbol :
HFE
reactivity :
rat
Usage :
ELISA, WB (1:1000 - 1:5000)
UsageText :
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for antiserum and 1-10 ug/ml for aff. pure IgG using ECL. ELISA: ~1:100000 using 50-100 ng control peptide/well.
Synonyms :
HFE, HFE1, HH, MHC class I-like protein HFE, Hemochromatosis, High Fe, MVCD7, HLA-H, HLAH, TFQTL2
SwissProtProtoID :
Q30201
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
Headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.