product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-PTGER3 / EP3
catalog :
LS-C23113
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human, rat
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
23695
AntibodyName :
LS-C23113
TargetSpecies :
rat
Host Species :
rabbit
Product Name :
Anti-PTGER3 / EP3
Specificity :
The N-terminal rat control peptide is conserved in all EP3 isoforms that differ at the C-terminus. It is 72% conserved in mouse EP3-beta and -gamma. No significant homology exist with either human EP3 or other EP (1, 2 or 4) receptors. Antibody cross-reactivity in various species is not established. The Control peptide, because of the small size (~3kD), is not suitable for Western. It should be used in ELISA or to neutralize antibody and confirm specificity of antibody. (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity.
ClonalityDesc :
polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
Neat serum, 40% glycerol.
ImmunogenDesc :
A 15 AA peptide within the 1st extracellular, N-terminal domain of Rat EP3 (1) coupled to KLH.
PurificationDesc :
Immunoaffinity Chromatography
RecommendedStorageDesc :
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
IsotypeName :
IgG
gene :
PTGER3 / EP3
StandardGeneSymbol :
PTGER3
gene family :
GPCR
Subfamily :
Prostanoid
reactivity :
rat
Usage :
ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)
UsageText :
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. EP3 is ~62kD protein in the kidney. ELISA: 1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Synonyms :
PTGER3, Ep3 prostanoid receptor, Ep3 receptor, EP3-I, EP3e, EP3-II, EP3-III, EP3-IV, PGE receptor, EP3 subtype, Prostaglandin receptor (PGE-2), Prostaglandin ep3 receptor, Prostanoid EP3 receptor, PGE receptor EP3 subtype, Pge2 receptor ep3, PGE2 receptor EP3 subtype, Prostaglandin E receptor 3, Prostaglandin e2 receptor ep3, EP3, Ep3 subtype pge2 receptor, PGE2-R, Prostaglandin E receptor EP3
SwissProtProtoID :
P43115
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
Headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.