product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-PTGER4 / EP4
catalog :
LS-C23118
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
product information
?AntibodyID :
23700
AntibodyName :
LS-C23118
TargetSpecies :
human
Host Species :
rabbit
Product Name :
Anti-PTGER4 / EP4
Specificity :
The human control peptide is 100% conserved in rabbit, 87% in canine, 94% in rat and mouse EP4. No significant homology exist with other EP (1, 2 or 3) receptors. Antibody cross-reactivity in various species is not established. The Control peptide, because of the small size (~3kD), is not suitable for Western. It should be used in ELISA or to neutralize antibody and confirm specificity of antibody. (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity.
ClonalityDesc :
polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
Neat serum, 40% glycerol.
ImmunogenDesc :
An 18 AA peptide within C-terminal, cytoplasmic domain 4 of Human EP4 (1) coupled to KLH.
PurificationDesc :
Immunoaffinity Chromatography
RecommendedStorageDesc :
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
IsotypeName :
IgG
gene :
PTGER4 / EP4
StandardGeneSymbol :
PTGER4
gene family :
GPCR
Subfamily :
Prostanoid
reactivity :
Human, Rabbit
Usage :
ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)
UsageText :
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. EP4 has been shown to be 47 and 78kD EP4 bands in the kidney. ELISA: 1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Synonyms :
PTGER4, EP4, EP4 prostaglandin receptor, EP4R, PGE receptor EP4 subtype, Prostanoid EP4 receptor, Prostaglandin E receptor 4, PGE receptor, EP4 subtype, PGE2 receptor EP4 subtype
SwissProtProtoID :
P35408
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
Headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.