This is a knockout-validated antibody summary, based on the publication "Comparative oncogenomics identifies tyrosine kinase FES as a tumor suppressor in melanoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgM
Company: Millipore
Antibody: v-Fes
Catalog number: OP02
Summary: Rat monoclonal IgM against v-Fes-transformed cells. Reacts with human, mouse and rat. Suitable for immunoprecipitation and neutralization studies.
Western blot
Fes+/+ or Fes–/– mouse melanoma lesions. Tissue samples were additionally homogenized with Precellys Homogenizer (Bertin Technologies) in protein lysis buffer (25 mM HEPES, pH 7.5, 0.3 M NaCl, 1.5 mM MgCl2 , 2 mM EDTA, 2 mM EGTA, 1 mM DTT, 1% Triton X-100, 10% glycerol, phosphatase/protease inhibitor cocktail).
5% milk in TBS, 0.2%Tween-20.
1:30,000 dilution.
HRP-conjugated secondary antibody (Cell Signaling Technology).
ECL Western blotting reagent (Thermo Scientific).
Western blot analysis of FES protein levels in Fes+/+ or Fes–/– melanoma lesions. Vinculin and GAPDH served as loading controls. Please see Figure 7c in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.