This is a knockout-validated antibody summary, based on the publication "Talin2-mediated traction force drives matrix degradation and cell invasion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: AbD Serotec

Antibody: talin2

Catalog number: MCA4770

Summary: Mouse monoclonal IgG1 against his-tagged polypeptide containing residues 489–911 of mouse talin1. Reacts with human and mouse protein. Suitable for western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Western blot | Immunocytochemistry

Control and talin1- or talin2-null (CRISPR mediated) U2OS cells.

WB: 1:1,000 dilution.

IC: 1:100 dilution.

IC: DyLight-488-conjugated goat anti-mouse IgG (H+L) (Thermo Scientific) or Dylight-550- or -633-labeled goat anti-mouse from Immunoreagents (Raleigh, NC).

IC: Images were acquired by using TIRF microscopy.

Please see Figure 2a,b in the article [1].

Talin1 (AbD Serotec, clone 97H6) is also KO validated in the article.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.