talin antibody | knockout validation | Sigma T3287
DOI
//dx.doi.org/10.13070/ko.en.6.1717
Date
2016-10-07

This is a knockout-validated antibody summary, based on the publication "Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

talin antibody | knockout validation | Sigma T3287 figure 1
Figure 1. Western blot of talin (Pan-talin) in control, talin-KO (TlnKo), kindlin-KO (KindKo) and keratinocyte (Kerat.) cells. GAPDH served as loading control. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Sigma

Antibody: talin

Catalog number: T3287

Summary: Mouse monoclonal IgG1 against purified chicken gizzard talin. Reacts specifically with mammalian and avian talin including, mouse, rat, human, chicken and with other species, like frog. Suitable for western blot, immunofluorescence and immunoprecipitation.

Validation Method

Western blot

Sample

talin-1 and -2 double knockout fibroblasts derived from mouse kidneys.

Primary incubation

1:1000 dilution

Secondary incubation

goat anti-mouse HRP (172-1011) (BioRad), 1:10,000

References
  1. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher