This is a knockout-validated antibody summary, based on the publication "Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Company: Abcam
Antibody: talin-2
Catalog number: ab105458
Summary: Mouse monoclonal against a recombinant protein corresponding to amino acids 489-911 of mouse talin 2. Reacts with mouse in western blot, immunoprecipitation, immunocytochemistry, immunofluorescence (frozen) and ELISA.
Western blot
talin-1 and -2 double knockout fibroblasts derived from mouse kidneys
1:2000 dilution
goat anti-mouse HRP (172-1011) (BioRad), 1:10,000
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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