talin-1 antibody | knockout validation | Abcam ab57758
DOI
//dx.doi.org/10.13070/ko.en.6.1718
Date
2016-10-07

This is a knockout-validated antibody summary, based on the publication "Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

talin-1 antibody | knockout validation | Abcam ab57758 figure 1
Figure 1. Western blot of talin-1 in control, talin-KO (TlnKo), kindlin-KO (KindKo) and keratinocyte (Kerat.) cells. GAPDH served as loading control. From [1].
Antibody information

Mouse monoclonal

Company: Abcam

Antibody: talin-1

Catalog number: ab57758

Summary: Mouse monoclonal against a recombinant protein corresponding to amino acids 1052-1150 of human talin. Reacts with mouse and human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry and flow cytometry.

Validation Method

Western blot

Sample

talin-1 and -2 double knockout fibroblasts derived from mouse kidneys.

Primary incubation

1:2000 dilution

Secondary incubation

goat anti-mouse HRP (172-1011) (BioRad), 1:10,000

References
  1. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher