This is a knockout-validated antibody summary, based on the publication "The ON:OFF switch, σ1R-HINT1 protein, controls GPCR-NMDA receptor cross-regulation: implications in neurological disorders", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of σ1R in the synaptosomes of cerebral cortex obtained from wild-type and σ1R−/− mice. NR1 served as loading control. From [1].

Rabbit polyclonal IgG

Company: Invitrogen

Antibody: sigma1R

Catalog number: 42-3300

Summary: Rabbit polyclonal IgG raised against a synthetic peptide derived from the C-terminal region of the rat sigma-1 receptor. Reacts with rat and mouse protein. Suitable for western blot, and it has been published its use in immunocytochemistry.

Western blot

Synaptosomes of cerebral cortex obtained from wild-type and σ1R−/− mice.

in Tris buffered saline (pH 7.7) + 0.05% Tween-20 overnight at 6°C.

for 2 h.

Chemiluminescence reagent (#170-5061, BioRad, Madrid, Spain) and recorded with a ChemiImager IS-5500 (Alpha Innotech, San Leandro, California).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.