This is a knockout-validated antibody summary, based on the publication "Cardiac tissue-restricted deletion of plakoglobin results in progressive cardiomyopathy and activation of {beta}-catenin signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of 12.5-day-old embryo lysates demonstrating the absence of PG protein in PG null embryos (Δ/Δ). GAPDH is used as loading control. From figure 1 [1].

Company: BD

Antibody: plakoglobin

Catalog number: 610253

Summary: Mouse IgG2a raised against human γ-catenin aa. 553-738. Reacts with human, mouse, rat, dog, and chicken proteins. It has been used in western blot, immunofluorescence, and immunoprecipitation.

Western Blot.

12.5-day-old embryo lysates homogenized in a modified radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA [ pH 8.0] , 1% NP-40, 0.5% Na deoxycholate, 0.1% sodium dodecyl sulfate (SDS) containing protease inhibitors and phosphatase inhibitor cocktails I and II (Roche Diagnostics) and centrifuged at 12,000 × g for 15 min.

blotting with an IRDye 680- or IRDye 800CW-conjugated secondary antibody (LI-COR)

Odyssey infrared imaging system (LI-COR), and quantitative densitometric analysis was performed by applying Odyssey version 1.2 infrared imaging software