pescadillo antibody | knockout validation | Santa Cruz sc-166300

This is a knockout-validated antibody summary, based on the publication "PES1 is a critical component of telomerase assembly and regulates cellular senescence", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

pescadillo antibody | knockout validation | Santa Cruz sc-166300 figure 1
Figure 1. PES1 WT or KO MCF7 cells (left) and MEFs (right) were transiently transfected with the indicated plasmids and immunoprecipitated with anti-Flag. The precipitates were used for assessment of Flag-h/mTERT, PES1, and β-actin expression by immunoblot. From [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: pescadillo

Catalog number: sc-166300

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 399-427 near the C-terminus of human pescadillo (PES1). Recommended for detection of pescadillo isoforms 1 and 2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and PES1-KO MCF7 and MEFs cell lines.

References
  1. Cheng L, Yuan B, Ying S, Niu C, Mai H, Guan X, et al. PES1 is a critical component of telomerase assembly and regulates cellular senescence. Sci Adv. 2019;5:eaav1090 pubmed publisher