This is a knockout-validated antibody summary, based on the publication "IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Imgenex, now part of Bio-techne.

Antibody: p73

Catalog number: NB100-56674, the same as the catalog number cited in the article, IMG-259A

Summary: Mouse monoclonal IgG1 (kappa light chain) against full-length human p73. The epitope is thought to lie around the center of the molecule. Reacts with alpha, beta, gamma and delta isoforms of mouse and human p73. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry and ChIP.

Western blot | Immunocytochemistry

Mouse embryo fibroblasts (MEFs) of WT; ΔNp73−/−; and p73−/− genotypes. Thymic lymphomas from ΔNfl/fl;p53−/−) or Ad-Cre-mCherry (ΔNp63Δ/Δ;p53−/− or ΔNp73Δ/Δ;p53−/−48 hours after infection with Adenovirus (Ad)-mCherry or Ad-Cre-mCherry.

1:250 dilution.

Horseradish peroxidase-conjugated antibodies at 1:5,000 dilution (Jackson Lab).

Using the ECL Plus Kit (Amersham) following the manufacturer’s protocol and x-ray autoradiography.

Please see Extended Data Figure 1d and 4d in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.