This is a knockout-validated antibody summary, based on the publication "NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure S7 in [1]. Western blot of lysates of wild-type and p65 knockout mouse embryonic fibroblasts probed with anti-p65 antibody. Actin serves as a loading control.

Mouse monoclonal

IgG1 kappa

Company: Santa Cruz Biotechnology

Antibody: NFkB p65

Catalog number: sc-8008

Summary: Mouse monoclonal antibody raised against residues 1-286 from the N-terminal region of human NFkB p65.

Supplier recommended for WB, IP, IF, IHC, flow cytometry and elisa.

Reacts with human, mouse and rat NFkB p65

Western blot

Lysates of wild-type and p65 -/- mouse embryonic fibroblasts.

Anti-p65 antibody.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.