This is a knockout-validated antibody summary, based on the publication "IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: Santa Cruz
Antibody: p63
Catalog number: 4A4
Summary: Mouse monoclonal IgG2a recommended for detection of p63 isoforms of mouse, rat and
human origin by western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry and ELISA.
Western blot
Western blot analysis in ΔNp63fl/fl;p53−/− MEFs before (ΔNp63fl/fl;p53−/−) and after (ΔNp63Δ/Δ;p53−/−) Ad-cre administration .
1:500 dilution.
Horseradish peroxidase-conjugated antibodies at 1:5,000 dilution (Jackson Lab).
Using the ECL Plus Kit (Amersham) following the manufacturer’s protocol and x-ray autoradiography.
Please see Extended Data Figure 5a in the article [1].
The product is no longer available at SCBT.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.