p62 Ick ligand antibody | knockout validation | BD Biosciences 610832

This is a knockout-validated antibody summary, based on the publication "The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p62 Ick ligand antibody | knockout validation | BD Biosciences 610832 figure 1
Figure 1. Immunoblots probed for mitochondrial components PINK1, SQSTM1, and TOMM20 after cells were treated with 1 μM oligomycin and antimycin A. From [1].
Antibody information

Mouse monoclonal IgG1, κ

Company: p62 Ick ligand

Antibody: BD Biosciences

Catalog number: 610832

Summary: Mouse monoclonal antibody against human p62 lck ligand (SQSTM1, aa. 257-437). Reacts with human. Suitable for western blot and immunofluorescence. Not recommended for immunohistochemistry or immunoprecipitation.

Validation Method

Western blot

Sample

WT and SQSTM1-KO iPSCs.

Primary incubation

Overnight at 4 C.

Secondary incubation

IR dye 680 RD donkey anti-mouse (LI-COR), for 1 h at room temperature.

Detection

Membranes were imaged on LI-COR Odyssey CLx.

References
  1. Poon A, Saini H, Sethi S, O Sullivan G, Plun Favreau H, Wray S, et al. The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons. Stem Cell Reports. 2021;16:1276-1289 pubmed publisher