This is a knockout-validated antibody summary, based on the publication "Disruption of Protein Processing in the Endoplasmic Reticulum of DYT1 Knock-in Mice Implicates Novel Pathways in Dystonia Pathogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: p58IPK
Catalog number: 2940
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human p58IPK. Reacts with human, mouse, and monkey. Suitable for western blot and immunoprecipitation.
Western blot
Cerebel from control and p58-KO mice. Tissues were lysed in RIPA buffer completed with N-ethylmaleimide and protease/phosphatase inhibitors.
HRP-linked goat anti-rabbit antibodies.
Chemidoc MP Imaging system (Biorad).
Western blot analysis in cerebellar tissue obtained from 3-week-old and 9-month-old mice for expression of the indicated proteins. Please see Figure 3b in the article [1].
- Beauvais G, Bode N, Watson J, Wen H, Glenn K, Kawano H, et al. Disruption of Protein Processing in the Endoplasmic Reticulum of DYT1 Knock-in Mice Implicates Novel Pathways in Dystonia Pathogenesis. J Neurosci. 2016;36:10245-10256 pubmed
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