This is a knockout-validated antibody summary, based on the publication "Glucocorticoid receptor triggers a reversible drug-tolerant dormancy state with acquired therapeutic vulnerabilities in lung cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative immunofluorescence images showing expression and localisation of GR (green), p57 (red) in p57-WT and p57-KO cell lines. DAPI is used as nuclear staining (blue) (n = 3). Scale bar, 10 μm. From [1].

Mouse monoclonal IgG2b κ

Company: Santa Cruz

Antibody: p57 Kip2

Catalog number: sc-56341

Summary: Mouse monoclonal antibody against full length human p57 Kip2. Recommended for detection of p57 Kip2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Immunocytochemistry

p57-WT and p57-KO A549, H2122 and H1944 cell lines.

1% BSA in PBS for 60 min.

1:50 dilution for 2 h at room temperature.

1:1,000 dilution goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A11001, Thermo Fisher Scientific).

Laser confocal microscopy (SP5, Leica).

The same clone (KP39) is sold as Santa Cruz Biotechnology sc-56341; LifeSpan Biosciences LS-C88548, LS-C88549, LS-B8635; Invitrogen MA5-14361.