This is a knockout-validated antibody summary, based on the publication "Regulation of PCGEM1 by p54/nrb in prostate cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of p54/nrb in control and p54/nrb knockout (KO) CWR22Rv1 cells with or without re-expression of p54/nrb. GAPDH was used as loading control. From [1].

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: p54/nrb

Catalog number: sc-376806

Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin. Recommended for detection of p54/nrb of mouse, rat, human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, canine, bovine and porcine.

Western blot

Control and p54/nrb knockout CWR22Rv1 cells.

Secondary antibodies conjugated with IRDye 800CW or IRDye 680 (LI-COR Biosciences, Lincoln, NE).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.