This is a knockout-validated antibody summary, based on publications "An important role for Myb-MuvB and its target gene KIF23 in a mouse model of lung adenocarcinoma", as cited below [1], and "A synergetic effect of BARD1 mutations on tumorigenesis" for western blot knockout validation (figure s3a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: p53

Catalog number: sc-99

Summary: Mouse monoclonal antibody against amino acids 14-389 of human p53. Recommended for detection of only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions of mouse, rat, and human origin by western blot and immunoprecipitation. Also reactive with additional species, including bovine and avian.

Western blot

Control and p53-null KPR8 cells.

Immunoblot of p53 before and after restoration in KPR8 cells. Please see Figure 5c in the article [1].

Technically is not a KO validation, but an indirect p53 induction due to deletion of another protein. The article uses p53-null cells instead of p53-KO cells.

The same clone (PAb 240) is sold as Novus Biologicals NB200-103, NBP2-80898; OriGene TA336582; Santa Cruz Biotechnology sc-99, sc-99 AC; Abcam ab26; Invitrogen 13-4100, AHO0112, MA5-15244; BD Biosciences 554167, 554166.