This is a knockout-validated antibody summary, based on the publication "The orphan nuclear receptor NR4A2 is part of a p53-microRNA-34 network", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 3 in [1]. Western blot of lysates of wild-type and p53 deleted HCT116 human colorectal carcinoma cells probed with anti-p53 antibody. Actin serves as a loading control.

Mouse monoclonal

Company: Santa Cruz Biotechnology

Antibody: p53

Catalog number: sc-65334

Summary: Mouse monoclonal antibody raised against recombinant human p53.

Supplier recommended for WB, IP, IF, IHC and elisa.

Reacts with human, mouse and rat p53.

Western blot

Lysates of wild-type and p53 deleted HCT116 human colorectal carcinoma cells.

Odyssey Blocking Buffer (Li-COR Biotechnology).

Anti-p53 antibody.

IRDye-conjugated goat anti-mouse IgG (LI-COR Technology).

LI-COR Odyssey Infrared Imaging System.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.