This is a knockout-validated antibody summary, based on the publication "IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting of p53 protein levels in HCT116 p53 (+/+), HCT116 p53 (−/−) treated with 5-FU (0.038 mM) for 24 hrs. Beta-actin served as loading control. From [1].

Goat polyclonal

Company: Santa Cruz Biotechnology

Antibody: p53

Catalog number: sc-6243-G

Summary: Goat polyclonal against an epitope mapping within amino acids 1-393 of full length p53 of human origin. Recommended for detection of p53 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA.

Western blot

HCT116 p53 (+/+) and HCT116 p53 (−/−) cells. Cells were lysed in lysis buffer containing 50 mM Tris-HCl (pH 8.0), 1% NP40, 250 mM NaCl, 50 mM NaF, 1 mM Na3VO4, 1 mM protease inhibitor (PMSF, aprotinin, leupeptin) and 1 mM DTT.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.