p53 antibody | knockout validation | Santa Cru Biotechnology sc-6243
DOI
//dx.doi.org/10.13070/ko.en.6.1848
Date
2016-11-23

This is a knockout-validated antibody summary, based on two publications "The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress" [1] and "HCT116 cells deficient in p21(Waf1) are hypersensitive to tyrosine kinase inhibitors and adriamycin through a mechanism unrelated to p21 and dependent on p53" [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: P53

Catalog number: sc-6243

Summary: Rabbit polyclonal antibody raised against an epitope within residues 1-393 of human p53. Supplier recommended for WB, IP, IF, IHC, flow cytometry and elisa. Reacts with mouse, rat and human p53.

Note: this antibody will be discontinued December 31, 2016.

Article "The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress"
Validation Method

Western blot

Sample

Lysates of murine embryonic fibroblasts.

Blocking agent

TBS containing 4% milk powder and 0.2% Tween 20.

Primary incubation

Anti-p53 antibody.

Secondary incubation

HRP-conjugated secondary antibody.

Detection

Chemiluminescent reagent.

Figure

See figure 3 in [1].

Article "HCT116 cells deficient in p21(Waf1) are hypersensitive to tyrosine kinase inhibitors and adriamycin through a mechanism unrelated to p21 and dependent on p53"
Validation Method

Western blot

Sample

HCT116p21−/− and HCT116p53−/− cells.

Detection

ECL system (GE Healthcare).

Figure

Please see Figure 5c in the article [2].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Jiang H, Jiang L, Wek R. The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress. J Biol Chem. 2007;282:3755-65 pubmed
  2. Ferrandiz N, Martin-Perez J, Blanco R, Donertas D, Weber A, Eilers M, et al. HCT116 cells deficient in p21(Waf1) are hypersensitive to tyrosine kinase inhibitors and adriamycin through a mechanism unrelated to p21 and dependent on p53. DNA Repair (Amst). 2009;8:390-9 pubmed publisher