This is a knockout-validated antibody summary, based on the publications "Importance of ROS-mediated autophagy in determining apoptotic cell death induced by physapubescin B", as cited below [1], and "TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling" for western blot knockout validation (figure 1b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. HCT116 p53+/+ (WT) and p53-/- (KO) cells were treated as indicated, cell lysate was collected and subjected to western blot to the indicated proteins. From [1].

Mouse monoclonal IgG1

Company: PharMingen

Antibody: p53

Catalog number: 554170

Summary: Mouse monoclonal IgG1 against a truncated recombinant human p53 fusion protein. Reacts with human by western blot, immunofluorescence, immunohistochemistry-formalin (antigen retrieval required), immunohistochemistry-frozen, and immunoprecipitation.

Western blot

p53+/+ and p53-/- HCT116 cells. Cells were lysed by the Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 20% glycerol, 2% SDS, 2 mM DTT, phosphatase inhibitor and proteinase inhibitor cocktail).

Blocking buffer (Thermo Scientific, 37538) for 30 min.

Enhanced chemiluminescence method (Thermo Scientific, 34076) and visualized by a Kodak Image Station 4000 R (Kodak).

The same clone (Pab 1801) is sold as Santa Cruz Biotechnology sc-98, sc-98 AC, sc-98 X; Abcam ab28; Invitrogen MA5-11296, 13-4000; BD Biosciences 554170, 554169.