This is a knockout-validated antibody summary, based on the publication "Dependence of p53-deficient cells on the DHX9 DExH-box helicase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of extracts from the indicated cell lines. Membranes are probed with antibodies indicated to the left. From [1].

Mouse monoclonal IgG1

Company: Novocastra

Antibody: p53

Catalog number: NCL-p53-505

Summary: Mouse monoclonal IgG1 against a recombinant protein corresponding to the full length wild type mouse p53. Reacts with mouse by western blot and immunohistochemistry.

Western blot

Protein extracts were prepared by lysing cells in RIPA lysis buffer (20 mM Tris-HCl [2, 3], 150 mM NaCl, 0.1% SDS, 1% NP40, 0.5% sodium deoxycholate, 1 mM β-glycerophosphate, 1 mM PMSF, 1 μg/ml leupeptin, 10 μg/ ml aprotinin, and 2.5 µM pepstatin A).

HRP-conjugated secondary anti-mouse (115-035-146, Jackson ImmunoResearch, West Grove, PA, USA).

Enhanced chemiluminescence (ECL) (Perkin Elmer, Waltham, MA, USA).