This is a knockout-validated antibody summary, based on the publication "The putative oncotarget CSN5 controls a transcription-uncorrelated p53-mediated autophagy implicated in cancer cell survival under curcumin treatment", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative western blot images in HCT116wt, HCT116p53−/− and HT29 cells treated with curcumin for 6 h. GAPDH was used as loading control. From [1].

Mouse monoclonal IgG2a

Company: Millipore

Antibody: p53

Catalog number: 05-224

Summary: Mouse monoclonal IgG2a against recombinant human wild type p53. Reacts with human protein by western blot, immunohistochemistry and immunoprecipitation.

Western blot

HCT116wt and HCT116p53−/− cells. Total proteins were extracted from the cells lysed in RIPA buffer.

5% non-fat milk in TBST for 1 h at room temperature.

For 2 h at room temperature.

HRP-conjugated anti-mouse IgG (Sigma, A9044) for 2 h at room temperature.

Enhanced chemiluminescence detection kit (ThermoFisher Scientific, Waltham, MA, USA) and developed on Kodak film.

The same clone (BP53-12) is sold as BioLegend 628201, 628202; Novus Biologicals NBP2-29453; OriGene TA327920; Santa Cruz Biotechnology sc-263; Abnova MAB4528, MAB11254; Rockland Immunochemicals 200-301-174; Immuno-Biological Laboratories 10041; Invitrogen MA1-19055, MA1-19551, A15733; Boster MA1078; US Biological P1001-26K, 138582; Sigma-Aldrich P5813; Cell Sciences MON7015; LifeSpan Biosciences LS-B431, LS-C11677, LS-C41085, LS-C45758, LS-C46336, LS-C95441, LS-C122264, LS-C153050, LS-C189571, LS-C358011, LS-C358559; EMD Millipore 05-224.