This is a knockout-validated antibody summary, based on the publication "Regulation of iron homeostasis by the p53-ISCU pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 1 in [1]. Western blot of lysates of wild-type and p53 -/- HCT116 human colorectal carcinoma cells probed with anti-p53 antibody. Actin serves as a loading control.

Mouse monoclonal

Company: Merck Millipore

Antibody: P53

Catalog number: OP43

Summary: Mouse monoclonal antibody raised against wild-type recombinant human p53. Recognises an epitope within residues 22-25 of human p53 sequence.

Supplier recommended for WB, IP and IHC.

Reacts with cat and human p53. Not recommended for rodent p53.

Western blot

Lysates of wild-type and p53 -/- HCT116 human colorectal carcinoma cells.

Anti-p53 antibody.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.