This is a knockout-validated antibody summary, based on the publication "Mitochondrial p32 is a critical mediator of ARF-induced apoptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: Lab Vision

Antibody: p53

Catalog number: DO1

Summary: Mouse monoclonal IgG2a against recombinant human wild-type p53. Reacts with human, mouse and primate protein by western blot, immunofluorescence, immunohistochemistry (paraffin) and ChIP.

Western blot

Saos2 and H1299 p53-deficient human cell lines and p19Arf−/−;p53−/− and p53−/− MEFs. Cells were lysed in 0.5% NP-40 buffer.

Please see Figure 7b,c in the article [1].

The same clone (DO-1) is sold as Bio-Rad MCA1701F, MCA1701PE, MCA1701, MCA1701B; BioLegend 645701, 645702; US Biological P1001-32C, P1001-26U1; Santa Cruz Biotechnology sc-126, sc-126 AC, sc-126 X, sc-126 HRP; LifeSpan Biosciences LS-C11553, LS-C87895, LS-C87898, LS-C131180, LS-C179709; Abcam ab80645; Active Motif 39553; Invitrogen MA5-12571, AHO0152; MyBioSource MBS212262; Sigma-Aldrich P6874, P9249, P9124; BD Biosciences 554293; EMD Millipore MABE327.