p53 antibody | knockout validation | Cell Signaling Technology 2527S

This is a knockout-validated antibody summary, based on the publications "Caspase-3 feedback loop enhances Bid-induced AIF/endoG and Bak activation in Bax and p53-independent manner", as cited below [1], and "Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination" for western blot knockout validation (figure 5j) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p53 antibody | knockout validation | Cell Signaling Technology 2527S figure 1
Figure 1. Western blot of p53 and Bax in the indicated cell lines. β-Actin was used as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling Technology

Antibody: p53

Catalog number: 2527S

Summary: Rabbit monoclonal IgG against full-length human p53 fusion protein. Reacts with human and monkey. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, flow cytometry and ChIP.

Validation Method

Western blot

Sample

WT and p53 KO HCT116 and DU145 cells.

Primary incubation

1:2,000 dilution

References
  1. Guo W, Zhang Y, Ling Z, Liu X, Zhao X, Yuan Z, et al. Caspase-3 feedback loop enhances Bid-induced AIF/endoG and Bak activation in Bax and p53-independent manner. Cell Death Dis. 2015;6:e1919 pubmed publisher
  2. Zheng H, Xu W, Zhou W, Yang R, Chen P, Liu T, et al. Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination. Cell Death Dis. 2021;12:497 pubmed publisher