This is a knockout-validated antibody summary, based on the publication "Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Cell Signaling
Antibody: p53
Catalog number: 9282
Summary: Rabbit polyclonal against full-length human p53 fusion protein. Reacts with human and monkey. Suitable for western blot, immunoprecipitation and ChIP.
Western blot
HCT-116 (KRAS mutant, p53+/+) and HCT-116 (KRAS mutant, p53−/−) cells. Whole-cell or tumour tissue extracts were prepared by radioimmunoprecipitation assay buffer (150 mM sodium chloride, 50 mM Tris pH 8.0, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with 10 mM NaF, 1 mM Na3VO4, 5 mM EDTA, 1 mM EGTA, 5 μg ml−1 leupeptin, 1 μg ml−1 pepstatin A, 1 mM phenylmethylsulfonyl fluoride, and protease and phosphatase inhibitor cocktail (Calbiochem).
5% bovine serum albumin for 1 h at room temperature.
1:1,000 dilution in Primary Antibody Dilution Buffer (Beyotime Biotechnology, China) at 4 °C overnight.
1:10,000 diluted IRDye 800CW Goat anti-Rabbit IgG (926–32211, LI-COR Biosciences).
Odyssey Infrared Imaging System (Odyssey, LI-COR).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.