This is a knockout-validated antibody summary, based on the publication "STIP is a critical nuclear scaffolding protein linking USP7 to p53-Mdm2 pathway regulation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot of p53 in U2OS (p53+/+), H1299 (p53−/−), HCT116 (p53+/+), and HCT116 (p53−/−) cells. Actin served as loading control. From [1].

Rabbit polyclonal IgG

Company: Bethyl Laboratories

Antibody: p53

Catalog number: A300-247A

Summary: Mouse monoclonal IgG against an epitope mapping between amino acid residues 50 and 100 of p53 of human origin. Recommended for detection of p53 of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry, ChIP-Seq, and PLA. Predicted to react with gorilla and chimpanzee due to 100% sequence identity.

Western blot

U2OS (p53+/+), H1299 (p53−/−), HCT116 (p53+/+), and HCT116 (p53−/−) cells. Whole cell lysates were prepared with M-PER buffer containing protease inhibitors (Pierce).

5% nonfat milk.

overnight at 4°C.

horseradish peroxidase-conjugated secondary antibodies (Santa Cruz).

ECL Kit (Pierce).

Authors do not specify which of the two antibodies (another one is SCBT sc-126) was used for the western blot. We assume both are KO validated.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.