This is a knockout-validated antibody summary, based on the publications "Constitutive p53 heightens mitochondrial apoptotic priming and favors cell death induction by BH3 mimetic inhibitors of BCL-xL", as cited below [1], and "4-Hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen" for western blot knockout validation (figure s1-1c) [2] and "Endogenous p53 expression in human and mouse is not regulated by its 3'UTR" for western blot knockout validation (figure s1-1c) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of p53 expression in HCT116 wt, p53−/− or p21−/− cells treated with ABT-737. From [1].

Mouse monoclonal IgG2b

Company: BD Pharmingen

Antibody: p53

Catalog number: 554294

Clone: DO-7. This clone is also sold as Bio-Rad MCA1703, BioLegend 645804, 645803, 645802, 645801, 645806, 645805, Dako M7001, LifeSpan Biosciences LS-C348578, LS-C312158, LS-C95342, LS-C202893, LS-C358016, LS-C11556, LS-C87890, LS-C87893, LS-C87894, Invitrogen MA5-12557, MA5-12554, MyBioSource MBS212266, Santa Cruz Biotechnology sc-47698, BioLogo PP100, Enzo Life Sciences BML-PW1095-0025, US Biological 138581, 168848, 168848-AF, Sigma-Aldrich SAB5100002, P8999, Leica Biosystems PA0067, NCL-p53-DO7-FITC, BD Biosciences 556534, Abnova MAB11246, and OriGene TA327719.

Summary: Mouse monoclonal IgG2b against human recombinant p53 protein. Reacts with human and monkey. Suitable for western blot, immunoprecipitation, flow cytometry,

Immunohistochemistry (formalin, antigen retrieval required) and immunohistochemistry (frozen).

Western blot

HCT116 wt, p53−/− and p21−/− cells. Cells were lysed in ice-cold lysis buffer (SDS 1% EDTA 10mM; Tris-HCl pH 8,1 50mM; protease inhibitors, Na3VO4 1mM, NaF 100 × ).

Chemiluminescence kit (Millipore).

The same clone (DO-7) is sold as Bio-Rad MCA1703; BioLegend 645801, 645802, 645803, 645804, 645805, 645806; Novus Biologicals NBP2-34308-0.1mg, NBP2-34605-0.1mg; OriGene CF804805, TA327719, TA804805, TA804805S; MilliporeSigma P8999; Santa Cruz Biotechnology sc-47698; LifeSpan Biosciences LS-C11556, LS-C95342, LS-C87890, LS-C87893, LS-C87894, LS-C202893, LS-C312158, LS-C339364, LS-C348578, LS-C358016; Abnova MAB11246; Enzo Life Sciences BML-PW1095-0025; Invitrogen MA5-12554, MA5-12557; Cell Signaling Technology 48818; Dako M7001; Agilent Technologies GA616; BD Biosciences 554294; Leica Biosystems PA0067, NCL-p53-DO7-FITC.