p53 antibody | knockout validation | Abcam ab77813

This is a knockout-validated antibody summary, based on the publication "Cooperation between liver-specific mutations of pten and tp53 genetically induces hepatocarcinogenesis in zebrafish", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p53 antibody | knockout validation | Abcam ab77813 figure 1
Figure 1. Western blot analyses were performed to confirm the expression of Pten, Tp53, pAkt, and Akt in liver tissues from 3-month-old fabp10WT (lanes 1–2) and pten;tp53 cKO fish (lanes 3–4). From [1].
Antibody information

Mouse monoclonal IgG1

Company: Abcam

Antibody: p53

Catalog number: ab77813

Summary: Mouse monoclonal antibody against recombinant full length p53 protein. Reacts with zebrafish by western blot.

Validation Method

Western blot

Sample

Control and tp53 cKO fish.

Primary incubation

1:2,000 dilution.

Secondary incubation

HRP-conjugated secondary antibodies.

Detection

SuperSignal West Pico kit (Thermo Fisher Scientific).

References
  1. Luo J, Lu C, Feng M, Dai L, Wang M, Qiu Y, et al. Cooperation between liver-specific mutations of pten and tp53 genetically induces hepatocarcinogenesis in zebrafish. J Exp Clin Cancer Res. 2021;40:262 pubmed publisher