This is a knockout-validated antibody summary, based on the publication "GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis" [1] and publication "Human EHMT2/G9a activates p53 through methylation-independent mechanism" [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. p53 protein levels were analyzed by immunoblotting after actinomycin D treatment in wild‐type and p53−/− HCT116 cells. Coomassie brilliant blue (CBB) staining serves as a loading control. Courtesy from the Authors [1].

Mouse monoclonal IgG2a

Company: Calbiochem

Antibody: p53 (Ab-6) (Pantropic)

Catalog number: OP43

Summary: Mouse monoclonal IgG2a against an epitope within amino acids 21-25 of recombinant human p53. Reacts with human and feline. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), and immunocytochemistry.

Western blot | Immunocytochemistry

Wild‐type and p53−/− HCT116 cells.

This is a knockout-validated antibody summary, based on the Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. p53 protein levels were analyzed by immunoblotting after actinomycin D treatment in wild‐type and p53−/− HCT116 cells. Coomassie brilliant blue (CBB) staining serves as a loading control. Courtesy from the Authors [1].

Mouse monoclonal IgG2a

Company: Calbiochem

Antibody: p53 (Ab-6) (Pantropic)

Catalog number: OP43

Summary: Mouse monoclonal IgG2a against an epitope within amino acids 21-25 of recombinant human p53. Reacts with human and feline. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), and immunocytochemistry.

Western blot

H1299 (p53−/−) and p53-recovered cells. Cells were incubated in lysis buffer (PBS, 50 mM NaCl, 1% NP-40, 1 mM PMSF) for 20 min at 4 °C and sonicated.

Western blot analysis of p53 in the indicated cell lines. β-actin was used as loading control. Please see Figure 1d,e in the article [2].

The same clone (DO-1) is sold as Bio-Rad MCA1701F, MCA1701PE, MCA1701, MCA1701B; BioLegend 645701, 645702; Santa Cruz Biotechnology sc-126, sc-126 AC, sc-126 X, sc-126 HRP; LifeSpan Biosciences LS-C11553, LS-C87895, LS-C87898, LS-C131180, LS-C179709; Abcam ab1101; Active Motif 39553; Invitrogen MA5-12571, AHO0152; MyBioSource MBS212262; Bethyl A500-015A; Sigma-Aldrich P6874, P9249, P9124; BD Biosciences 554293; EMD Millipore MABE327, OP43.