This is a knockout-validated antibody summary, based on the publication "FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
rabbit polyclonal
Company: Cell Signaling Technology
Antibody: P44/42 MAPK
Catalog number: 9102
Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of rat p44MAPK. Recognises total p44/42 MAPK. Supplier recommended for WB, IP, IHC, IF and flow cytometry. Reacts with human, mouse, rat, hamster, monkey, mink, bovine, Zebrafish, Sacharomyces cerevisiae and bovine p44/42 MAPK.
Western blot
Whole cell lysates of wild-type and Erk2 -/- (Erk2 = p42 MAPK) mouse embryonic stem cells.
1:1000 dilution.
Peroxidase conjugated anti-rabbit IgG (Sigma, A6154) at 1:3000 dilution.
Super Signal West (Pierce).
See figure 4 from the article [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- Kunath T, Saba-El-Leil M, Almousailleakh M, Wray J, Meloche S, Smith A. FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment. Development. 2007;134:2895-902 pubmed
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