p38 MAPK beta antibody | knockout validation | Life Technologies 33-8700

This is a knockout-validated antibody summary, based on the publication "Loss of Functionally Redundant p38 Isoforms in T Cells Enhances Regulatory T Cell Induction", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p38 MAPK beta antibody | knockout validation | Life Technologies 33-8700 figure 1
Figure 1. Naïve CD4+ T cells from the indicated mice were left unstimulated or stimulated with anti-CD3 and anti-CD28. Whole cell lysates were prepared after the indicated durations of stimulation and analyzed by immunoblotting. From [1].
Antibody information

Mouse monoclonal IgG1/IgG2a

Company: Life Technologies

Antibody: p38 MAPK beta

Catalog number: 33-8700

Summary: Mouse monoclonal IgG1/IgG2a against recombinant, full-length human p38 beta-2; the product of the p38-beta gene. Reacts with human and mouse, and has been published to react with rabbit. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

Naïve CD4+ T cells obtained from spleen of wildtype, p38α-KO, p38β-KO and p38α/β-DKO mice.

References
  1. Hayakawa M, Hayakawa H, Petrova T, Ritprajak P, Sutavani R, Jiménez-Andrade G, et al. Loss of functionally redundant p38 isoforms in T cells enhances regulatory T cell induction. J Biol Chem. 2016;: pubmed publisher