This is a knockout-validated antibody summary, based on the publication "Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot for p38α of DN3 thymocytes from WT and p38α conditional KO (p38cKO) mice. Actin is shown as a loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: p38 MAPK

Catalog number: sc-7972

Summary: Mouse monoclonal IgG1 (kappa light chain) raised against amino acids 213-360 mapping at the C-terminus of p38α of human origin. Recommended for detection of p38α and p38β of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA. Also reactive with additional species, including and equine and porcine.

Western blot

DN3 thymocytes from WT and p38α conditional KO mice, lysed in Triton lysis buffer (20 mM Tris (pH 7.4), 137 mM NaCl, 2 mM EDTA, 1% Triton X-100, 10% glycerol, 25 mM β-glycerophosphate and 1 mM sodium vanadate) supplemented with mammalian protease inhibitor cocktail (Sigma).

1:1,000

anti-mouse HRP (Jackson ImmunoResearch Laboratories 115-035-166, 1:10,000)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.