This is a knockout-validated antibody summary, based on the publication "Statin-induced depletion of geranylgeranyl pyrophosphate inhibits cell proliferation by a novel pathway of Skp2 degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 1 in [1]. Western blot of lysates of wild-type (+/+) and p27 knockout (-/-) MEFs probed with anti-p27 antibody. Tubulin acts as a loading control.

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: p27

Catalog number: sc-528

Summary: Rabbit polyclonal antibody raised against a peptide sequence corresponding to the C-terminal region of human p27.

Supplier recommended for WB, IP, IF, IHC and elisa.

Reacts with human, mouse, rat, dog, cat, cow and pig p27.

Note: this antibody was discontinued on December 15, 2016.

Western blot

Lysates of wild-type and p27 knockout mouse embryonic fibroblasts.

Antip27 antibody.

ECL reagent.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.