p27 antibody | knockout validation | BD Transduction 610242

This is a knockout-validated antibody summary, based on the publication "p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes", as cited below [1], and "Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment" (Western blot, supplementary figure 1d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p27 antibody | knockout validation | BD Transduction 610242 figure 1
Figure 1. p27−/− MEFs were infected with an empty vector or with a vector harboring full length p27WT. Levels of p27 in these cells were determined by WB. Actin was used as loading control. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Transduction

Antibody: p27

Catalog number: 610242

Summary: Mouse monoclonal IgG1 against mouse Kip1 aa. 1-197. Reacts with human, dog, rat, mouse, chicken, and frog. Suitable for western blot, immunoprecipitation, immunofluorescence and immunohistochemistry.

Validation Method

Western blot

Sample

WT and p27−/− MEFs.

Notes

P21 (sc-6246) is also KO validated in Fig. 7i.

References
  1. Orlando S, Gallastegui E, Besson A, Abril G, Aligué R, Pujol M, et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res. 2015;43:6860-73 pubmed publisher
  2. Ding L, Shunkwiler L, Harper N, Zhao Y, Hinohara K, Huh S, et al. Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment. PLoS Genet. 2019;15:e1008002 pubmed publisher