p21 antibody | knockout validation | Sigma P1484
DOI
//dx.doi.org/10.13070/ko.en.6.1888
Date
2016-11-30

This is a knockout-validated antibody summary, based on the publication "HCT116 cells deficient in p21(Waf1) are hypersensitive to tyrosine kinase inhibitors and adriamycin through a mechanism unrelated to p21 and dependent on p53", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2b

Company: Sigma

Antibody: p21

Catalog number: P1484

Summary: Mouse monoclonal IgG2b against recombinant human p21WAF1/Cip1. Reacts with human, and mouse protein. Suitable for western blot, immunoprecipitation, ELISA and microarray.

Validation Method

Western blot

Sample

HCT116p21−/− and HCT116p53−/− cells.

Detection

ECL system (GE Healthcare).

Figure

Please see Figure 5c in the article [1].

Clone note

The same clone (CP74) is sold as LifeSpan Biosciences LS-C88545, LS-C88546, LS-B8634; Sigma-Aldrich P1484; EMD Millipore 05-655.

References
  1. Ferrandiz N, Martin-Perez J, Blanco R, Donertas D, Weber A, Eilers M, et al. HCT116 cells deficient in p21(Waf1) are hypersensitive to tyrosine kinase inhibitors and adriamycin through a mechanism unrelated to p21 and dependent on p53. DNA Repair (Amst). 2009;8:390-9 pubmed publisher