This is a knockout-validated antibody summary, based on publications "p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes" [1] for western blot in WT and p21^−/− MEFs (see below for detailed discussion) and "p21(CIP1/WAF1)-dependent inhibition of cardiac hypertrophy in response to Angiotensin II involves Akt/Myc and pRb signaling" [2] for western blot in tissue lystes from p21 knockout mice and p21 reconstituted mice, and "Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability" [3] for knockout-validation for western blot and immunohistochemistry (see figure 1c and 5a in the article). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. p27−/− MEFs were infected with an empty vector or with a vector harboring full length p27WT. Levels of p27 in these cells were determined by WB. Actin was used as loading control. From [1].

Mouse monoclonal IgG2b

Company: Santa Cruz

Antibody: p21

Catalog number: sc-6246

Summary: A mouse monoclonal IgG2b provided at 200 µg/ml; raised against amino acids 1-159 representing full length p21 of mouse origin; recommended for detection of p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; available conjugated to agarose (sc-6246 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-6246 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-6246 PE), fluorescein (sc-6246 FITC), Alexa Fluor® 488 (sc-6246 AF488) or Alexa Fluor® 647 (sc-6246 AF647), 200 µg/ml, for IF, IHC(P) and FCM; available conjugated to biotin (sc-6246 B), 200 µg/ml, for WB, IHC(P) and ELISA; and to either TRITC (sc-6246 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-6246 AF405, 100 µg/2 ml), for IF, IHC(P) and FCM

Western blot; Western blot and immunohistochemistry in [3] (see figure 1c and 5a in the article)

WT and p21^−/− MEFs.