p21 antibody | knockout validation | Cell Signaling Technology 2947S

This is a knockout-validated antibody summary, based on the publication "Akt inhibition improves irinotecan treatment and prevents cell emergence by switching the senescence response to apoptosis", as cited below [1]. This antibody was also validated for Western blot in the article "Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy" (Figure 7b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p21 antibody | knockout validation | Cell Signaling Technology 2947S figure 1
Figure 1. Figure 6. From [1]. Western blot with p21 antibody 2947 on the lysates of HCT116 and HCT116 p21−/− cells.
Antibody information

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: p21

Catalog number: 2947S

Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding to a sequence within the C-terminal region of human p21.

Supplier recommended for WB, IP, IF and IHC.

Reacts with human and monkey p21.

Validation Method

Western blot

Sample

Lysates of wild-type and p21 -/- HCT116 human colorectal carcinoma cells.

Blocking agent

TBS containing 5% BSA and 0.1% Tween 20.

Primary incubation

Anti-p21 antibody diluted 1:1000 in blocking buffer.

Secondary incubation

HRP-conjugated secondaty antibody (Santa Cruz Biotechnology).

Detection

Chemiluminescence reagent with BioRad ChemiDoc XRS imaging device.

References
  1. Vétillard A, Jonchère B, Moreau M, Toutain B, Henry C, Fontanel S, et al. Akt inhibition improves irinotecan treatment and prevents cell emergence by switching the senescence response to apoptosis. Oncotarget. 2015;6:43342-62 pubmed publisher
  2. Le Duff M, Gouju J, Jonch re B, Guillon J, Toutain B, Boissard A, et al. Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy.. Cell Death Dis. 2018;9:199 pubmed publisher