p21 antibody | knockout validation | Cell Signaling Technology 2946
DOI
//dx.doi.org/10.13070/ko.en.6.1902
Date
2016-11-30

This is a knockout-validated antibody summary, based on the publication "Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2

Company: Cell Signaling Technology

Antibody: p21

Catalog number: 2946

Summary: Mouse monoclonal IgG2a recombinant amino-terminal portion of human p21. Reacts with human protein by western blot and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

WT and p21−/− cells.

Secondary incubation

HRP-conjugated secondary anti-mouse IgG (G21040; Molecular Probes).

Detection

WesternBright ECL HRP substrate (K-12045-D50; Advansta).

Figure

Please see Figure 1c in the article [1].

Clone note

The same clone (DCS60) is sold as Santa Cruz Biotechnology sc-56335; LifeSpan Biosciences LS-C179603; Abnova MAB2028; Invitrogen MA1-12303; Cell Signaling Technology 2946.

References
  1. Overton K, Spencer S, Noderer W, Meyer T, Wang C. Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states. Proc Natl Acad Sci U S A. 2014;111:E4386-93 pubmed publisher