p190-A RhoGAP antibody | knockout validation | Cell Signaling 12164

This is a knockout-validated antibody summary, based on the publication "p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

p190-A RhoGAP antibody | knockout validation | Cell Signaling 12164 figure 1
Figure 1. Western blotting of the indicated proteins in WCLs from Ishikawa cells with p190A KO by CRISPR-Cas9 methods. Parental Ishikawa cells were used as a control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: p190-A RhoGAP

Catalog number: 12164

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Asn585 of human p190-A RhoGAP protein. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and p190A KO Ishikawa cells.

Primary incubation

1:1,000 dilution.

References
  1. Wen X, Wan J, He Q, Wang M, Li S, Jiang M, et al. p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer. Signal Transduct Target Ther. 2020;5:81 pubmed publisher