p130 Cas antibody | knockout validation

p130 Cas antibody | knockout validation | Cell Signaling 13846

This is a knockout-validated antibody summary, based on the publication "BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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p130 Cas antibody | knockout validation | Cell Signaling 13846 figure 1

Figure 1. Western blotting results showing successful BCAR1-KO in H1975 and H1299 cells. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: p130 Cas

Catalog number: 13846

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Leu116 in the SH3 domain of human p130 Cas (BCAR1) protein. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence (immunocytochemistry) and flow cytometry.

Validation Method

Western blot

Sample

Control and BCAR1-KO H1975 and H1299 cells.

Primary incubation

1:1,000 dilution.

References

Mao C, Jiang S, Wang X, Tao S, Jiang B, Mao C, et al. BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma. Int J Biol Sci. 2021;17:2461-2475 pubmed publisher

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