This is a knockout-validated antibody summary, based on the publication "Rac1-mediated membrane raft localization of PI3K/p110β is required for its activation by GPCRs or PTEN loss", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Wt, p110α,β-KO (DKO), DKO+p110α-wt and DKO+p110β-wt MEFs were analyzed in immunoblots for expression of p110α and p110β. Actin was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: p110beta

Catalog number: 3011

Summary: Rabbit monoclonal IgG against a synthetic peptide of human PI3K p110β. Reacts with human protein by western blot, and immunoprecipitation.

Western blot

Wt, p110α,β-KO (DKO), DKO+p110α-wt and DKO+p110β-wt MEFs.

5% non-fat milk (LabScientific, Highlands, New Jersey) in TBS for 30 min.

Diluted in TBS containing 5% milk and 0.1% Tween-20 overnight at 4°C.

IRDye 800CW goat anti-rabbit secondary antibodies (Li-Cor, Lincoln, Nebraska) diluted in 5% milk-TBS for 1.5 hr at RT.

Odyssey CLx Scanner.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.