This is a knockout-validated antibody summary, based on the publication "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Optn in primary MEFs isolated from WT or OPTN470T mice after infection with Sendai virus (SeV) for the indicated times. Western blot of Optn in BMDMs isolated from WT or OPTN470T mice stimulated with poly(I:C) for the indicated times. Western blot of Optn in WT or OPTN knockout HeLa cells after infection with Sendai virus (SeV) for 6 or 8 h. GAPDH was used as loading control. From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: optineurin

Catalog number: ab23666

Summary: Rabbit polyclonal IgG against a synthetic peptide corresponding to amino acids 575-591 of human Optineurin. Recommended for detection of Optineurin of mouse, rat and human origin by western blot and immunofluorescence/immunocytochemistry. Predicted to work with cow, pig, and monkey.

Western blot

Primary MEFs and BMDMs isolated from WT or OPTN470T mice and WT or OPTN knockout HeLa cells. Cells were lysed in lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 % Triton X-100, 2 mM EDTA, 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM sodium orthovanadate) supplemented with protease inhibitor cocktail (Thermo Scientific).

1:5,000 dilution.

Chemiluminescence (Immobilon Western, Merck Millipore, Billerica, MA, USA).