This is a knockout-validated antibody summary, based on the publication "Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Stathmin was immunoprecipitated from sciatic nerve extracts of 34-day-old mice. Western blot shows from left to right, input (Ip) and eluate (E) from wild-type and Nefl−/− mice nerve extracts after anti-IgG control and anti-stathmin pulldown. From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: neurofilament-light chain

Catalog number: ab9035

Summary: Rabbit polyclonal IgG against pig intermediate filaments from spinal cords. Reacts with pig and rat, and is predicted to work with bird, and all mammals. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen).

Western blot

Wild-type and Nefl−/− mice nerve extracts. Tissues were lysed in IP buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1 % Triton X-100, 2 mM EDTA, Protease inhibitor, 1 mM NaF, 10 mM sodium pyrophosphate, 1 µM okadaic acid, 2 mM sodium orthovanadate) using a glass–glass homogenizer.

ECL or ECL advance (GE Healthcare).