This is a knockout-validated antibody summary, based on the publication "Neuronal nitric oxide synthase localizes to utrophin expressing intercalated discs and stabilizes their structural integrity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal IgG

Company: Santa Cruz Biotechnology

Antibody: nNOS

Catalog number: sc-648

Summary: Rabbit polyclonal IgG against an epitope mapping near the C-terminus of NOS1 of rat origin. Recommended for detection of NOS1 (ncNOS) of mouse, rat, and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine, canine, bovine, porcine and avian.

Immunohistochemistry

Quadriceps from WT, mdx, and nNOSμ KO mice. Tissue was dissected out and frozen in OCT for cryosectioning.

1:1,000 dilution.

Images were taken using a Nikon Eclipse T1 or Carl Zeiss AxioVert 200M microscope with a 40× or 20× objective and processed using NIS-Elements BR or Carl Zeiss AxioVision software.

Please see Figure 1c in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.