This is a knockout-validated antibody summary, based on the publications "Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons", as cited below [1], and "The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice" for (figure 1c,s4e) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of brain membrane proteins from WT and Slo2.2 KO mice. Tubulin was used as loading control. From [1].

Antibody: mSlo2.2

Catalog number: 75-051

Summary: Mouse monoclonal raised against a fusion protein mapping to amino acids 1168-1237 of rat Slo2.2. It reacts with human (weak), mouse, and rat. Recommended use in western blot and immunohistochemistry.

Company: Antibodies inc., distributing for Neuromab.

Western blot

Brain membrane proteins from WT, Slo2.1 KO, and Slo2.2 KO mice.

5% nonfat milk in Tris-buffered saline-Tween 20 solution (pH 7.3) at room temperature for 1 hr

10 ug/ml, overnight incubation at 4C

Blocking solution containing mouse Trueblot Ultra HRP-conjugated anti-mouse IgG (at 1:2000 dilution, eBioscience, San Diego, CA, United States) at room temperature for 1 hr.

Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Pittsburgh, PA, United States)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

The same clone (N3/26) is sold as OriGene 73-051, 75-051; Abcam ab94578; Invitrogen MA5-27609, MA5-45434, MA5-45435, MA5-45436, MA5-45437; Neuromab 73-051, 75-051.