This is a knockout-validated antibody summary, based on the publication "Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: Antibody characterisation and a semi-quantitative western blot study", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure 2 in [1] Western blot probed with anti-mGlu3 antibody. Lanes 1 and 3: WT mouse brain. Lane 2: mGlu2/3 double KO mouse brain. Lane 4: mGlu3 KO mouse brain.

Rabbit monoclonal

Company: Abcam

Antibody: mGlu3

Catalog number: ab166608 (Clone EPR9009(2))

Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding to a region within human mGlu3.

Recombinant rabbit monoclonal antibody.

Supplier recommended for WB.

Reacts with human, mouse and rat mGlu3.

Western blot

Brain lysates from wild-type, mGlu2/3 double knockout and mGlu3 knockout mice.

PBS containing 5% milk powder and 0.1% Tween.

Anti-mGlu3 antibody diluted 1:50000 in PBS containing 2% milk powder and 0.1% Tween.

ECL reagent.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.